Shinto Kunjamma John
Veterinary Microbiology Technician
Curriculum Vitae:
- 2005–present: Veterinary Microbiology Technician, Institute of Zoology, UK.
- 1998–2005: Laboratory Technician, Fahad bin Sultan Falcon Center, Saudi Arabia.
- 1995–1998: Laboratory Technician, Cardio-Thoracic Science Center Blood Bank, India.
- 1994–1995: Laboratory Technician, Holy Family Hospital, India.
Current Projects:
My work comprises the collection, isolation and identification of microorganisms, anti-biograms, from both the clinical and post-mortem examination specimens from various Wildlife Epidemiology Theme (WLE) projects and assisting in post-mortem examinations of wildlife. I maintain isolation and identification techniques for the Garden Wildlife Health (GWH) project, including isolating and identifying Trichomonassp. I conduct isolation and identification techniques for the Cetacean Strandings Investigation Programme (CSIP) , which monitors disease in cetaceans, pinipeds, basking sharks & turtles around the British Isles.
The microbiology laboratory also screens captive exotic animals in quarantine and from within the collections at London Zoo and Whipsnade Zoo. All reintroduction projects authorised by Natural England include a detailed veterinary management protocol. This consists of screening, clinical & post-mortem examination of endangered indigenous British species including red squirrel (Sciurus vulgaris), corncrake (Crex crex), red kite (Milvus milvus) common dormouse (Muscardinus avellanarius), field cricket (Gryllus campstris), red-barbed ants (Formica rufibarbis) and cirl bunting (Emberiza cirlus).
The laboratory provides technical support for molecular diagnostic studies of various amphibian species, looking for the chytrid fungus (Batrachochytrium dendrobatidis) and Ranavirus using Real-time PCR.
Finally, I manage several wildlife pathology archives (including frozen and formalin tissues and parasite samples) in posterity.
Research Interests:
My research interests include isolating and identifying pathogenic microorganisms from wild animal population and comparative studies of micro-flora in wild-caught and captive-bred animals.
Our screening methods have led to advances in the practice of ‘parasite conservation’, where captive-bred animals destined for release are managed so that their natural complement of bacteria, viruses, worms and fungi are as similar to their wild cousins as possible. This reduces the risk of them encountering novel pathogens after release. It also reduces the need for medication during captivity which has been linked to resistance in parasites. These advances have led to improved veterinary protocols in breeding projects across the UK.
I am also interested in raptors and have studied haematozoa of falcons, normal and abnormal haematology of raptors and the haemo-response to therapeutics and disease in birds of prey.
Publications, Theses and Reports:
Wilkinson V, Rodriguez-Ramos Fernandez J, Núñez A, Macgregor SK, John SK, Dallman TJ, Cunningham AA, de Pinna EM, Lawson B. Novel Salmonella Variant Associated with Mortality in Two Great Spotted Woodpeckers (Dendrocopos major). Journal of Wildlife Diseases 2019; in press.
Katharina Seilern-Moy, Julia Rodriguez-Ramos Fernandez, Shaheed K. Macgregor, Shinto K. John, Chris Linton, Andrew A. Cunningham and Becki Lawson. (2019) Fatal phaeohyphomycosis due to Exophiala sp. infection in a free-living common toad Bufo bufo. Diseases of Aquatic Organisms 133: 19–24, https://doi.org/10.3354/dao03341.
Saldanha IF, Lawson B, Goharriz H, Rodriguez-Ramos Fernandez J, John SK, Fooks AR, Cunningham AA, Johnson N, Horton DL (2019). Extension of the known distribution of a novel clade C betacoronavirus in a wildlife host. Epidemiology and Infection 1–8. https://doi.org/10.1017/ S0950268819000207.
Helle B. Hydeskov, Corinne F.L. Amar, Julia Rodriguez-Ramos Fernandez, Shinto K. John, Shaheed K. Macgregor, Andrew A. Cunningham, and Becki Lawson. (2019) Listeria monocytogenes infection of free-living western European Hedgehogs (Erinaceus Europaeus). Journal of Zoo and Wildlife Medicine 50(1) 183–189. https://doi.org/10.1638/2018-0093.
Gabriela Peniche, Julia Rodriguez-Ramos Fernandez, Chris Durrant, Shinto K. John, Shaheed K. Macgregor, Andrew A. Cunningham & Becki Lawson. (2017) Nested PCR for Suttonella ornithocola reveals widespread infection in British Paridae species. European Journal of Wildlife Research 63: 50 DOI 10.1007/s10344-017-1105-6.
Becki Lawson, Lydia H. V. Franklinos, Julia Rodriguez-Ramos Fernandez, Clare Wend-Hansen, Satheesh Nair, Shaheed K. Macgregor, Shinto K. John, Romain Pizzi, Alejandro Núñez, Philip M. Ashton, Andrew A. Cunningham & Elizabeth M. de Pinna (2018). Salmonella Enteritidis ST183: emerging and endemic biotypes affecting western European hedgehogs (Erinaceus europaeus) and people in Great Britain. Scientific Reports 8: 2449, DOI:10.1038/s41598-017-18667-2.
Becki Lawson, Robert A. Robinson, Julia Rodriguez-Ramos Fernandez, Shinto K. John, Laura Benitez, Conny Tolf, Kate Risely, Mike P. Toms, Andrew A. Cunningham & Richard A. J. Williams. (2018) Spatio-temporal dynamics and aetiology of proliferative leg skin lesions in wild British finches. Scientific Reports 8: 14670, DOI:10.1038/s41598-018-32255-y.
Zu Ermgassen, E., Durrant, C., John, S., Gardiner, R., Alrefaei, A. F., Cunningham, A. A. & Lawson, B. (2016) Evidence for aclinical carriage of the European epidemic strain of Trichomonas gallinae in finches, but not other non-columbiforms. Parasitology 143(10), 294–300. DOI: 10.1017/S0031182016000780.
Franklinos, L.H.V., Efstratiou, A., Macgregor, S.K., John, S.K., Hopkins, T., Cunningham, A.A. & Lawson, B. (2015) Streptococcus pyogenes infection in a free-living European hedgehog (Erinaceus europaeus). EcoHealth 12(4), 689–92. DOI: 10.1007/s10393-015-1051-2.
Beckmann, K.M., Borel, N., Pocknell, A.M., Dagleish, M.P., Sachse, K., John, S.K., Pospischil, A., Cunningham, A.A. & Lawson, B. (2014) Chlamydiosis in British garden birds (2005–2011): retrospective diagnosis and Chlamydia psittaci genotype determination. EcoHealth 11(4), 544–563. DOI:10.1007/s10393-014-0951-x.
Beckmann, K.M., Harris, E., Pocknell, A.M., John, S.K., Macgregor, S.K., Cunningham, A.A. & Lawson, B. (2014) Aprocta cylindrica (Nematoda) infection in a European robin (Erithacus rubecula) in Britain. Journal of Wildlife Diseases 50(4), 986-9. DOI: 10.7589/2014-02-035.
Lawson, B., de Pinna, E., Horton, R.A., Macgregor, S.K., John, S.K., Chantrey, J., Duff, J.P., Kirkwood, J.K., Simpson, V.R., Robinson, R.A., Wain, J. & Cunningham, A.A. (2014) Epidemiological evidence that garden birds are a source of human salmonellosis in England and wales. PLoS ONE 9 (2), e88968. DOI:10.1371/journal.pone.0088968.
Chi, J.F-C., Lawson, B., Durrant, C., Beckmann, K., John, S., Alrefaei, A.F., Kirkbride, K., Bell, D., Cunningham, A.A. & Tyler, K. (2013) The finch epidemic strain of Trichomonas gallinae is predominant in British non-passerines. Parasitology 140, 1234–1245. DOI: 10.1017/S0031182013000930.
Colvile, K.M., Lawson, B., Pocknell, A.M., John, S.K., Dagleish, M.P. & Cunningham, A.A. (2012) Chlamydiosis in British songbirds. Veterinary Record. 171, 177. DOI: 10.1136/vr.100506.
Lawson, B., Cunningham, A.A., Chantrey, J., Hughes, L.A., John, S.K., Bunbury, N., Bell, D.J. & Tyler, K.M. (2011) A clonal strain of Trichomonas gallinae is the aetiologic agent of an emerging avian epidemic disease. Infection, Genetics and Evolution. 11, 1638–1645. DOI:10.1016/j.meegid.2011.06.007.
Lawson, B., Hughes, L.A., Peters, T., de Pinna, E., John, S.K., Macgregor S.K. & Cunningham, A.A. (2011) Pulsed-field gel electrophoresis supports the presence of host-adapted Salmonella Typhimurium strains in the British garden bird population. Applied and Environmental Microbiology 77, 8139–8144. DOI:10.1128/AEM.00131-11.
Lawson, B., Robinson, R.A., Neimanis, A., Handeland, K., Isomursu, M., Agren, E.O., Hamnes, I.S., Tyler, K.M., Chantrey, J., Hughes, L.A., Pennycott, T.W., Simpson, V.R., John, S.K., Peck, K.M., Toms, M.P., Bennett, M., Kirkwood, J.K. & Cunningham, A.A. (2011) Evidence of spread of the emerging infectious disease, finch trichomonosis, by migrating birds. EcoHealth 8, 143–153. DOI: 10.1007/s10393-011-0696-8.
Lawson, B., Malnick, H.M., Pennycott, T.W., Macgregor, S.K., John, S.K., Duncan, G., Hughes, L.A., Chantrey, J. & Cunningham, A.A. (2010) Acute necrotising pneumonitis associated with Suttonella ornithocola infection in tits (Paridae).Veterinary Journal 188, 96–100. DOI: 10.1016/j.tvjl.2010.03.010.
Robinson, R.A., Lawson, B., Toms, M.P., Peck, K., Kirkwood, J.K., Chantrey, J., Clatworthy, I., Evans, A., Hughes, L., Hutchinson, O.C., John, S., Pennycott, T., Perkins, M., Rowley, P., Simpson, V.R., Tyler, K. & Cunningham, A.A. (2010) Emerging Infectious Disease leads to Rapid Population Decline of Common British Birds. PLoS ONE. 5(8), e12215. DOI:10.1371/journal.pone.0012215.
Samour, J.H., Naldo, J.L. & John, S.K. (2006) Normal haematological values in captive Gyr falcons (Falco rusticolus). Falco 28, 22.
Samour, J.H., John, S.K. & Naldo, J.L. (2005) Endoparasites in falcons in the Kingdom of Saudi Arabia. Exotic DVM Veterinary Magazine, Volume 6.6, February/ March 2005.
Samour, J.H., Naldo, J.L. & John, S.K. (2005) Normal haematological values in captive Gyr falcons (Falco rusticolus). Veterinary Record 157, 844–846.
Samour, J.H., Naldo, J.L. & John, S.K. (2005) Therapeutic management of Babesia shortii infection in a peregrine falcon (Falco peregrinus). Journal of Avian Medicine and Surgery 19, 294–296.
Samour, J.H., Naldo, J.L. & John, S.K. (2001) Staining characteristics of the eosinophil in the Saker falcon (Falco cherrug). Exotic DVM Veterinary Magazine, Volume 3.4, August/ September 2001.